RESEARCH ARTICLE


Hydrogen Peroxide and Quercetin Induced Changes on Cell Viability, Apoptosis and Oxidative Stress in HepG2 Cells



Ayşe Mine Yılmaz1, 2, Gökhan Biçim1, 2, Kübra Toprak3, Betül Karademir Yılmaz1, 2, Irina Milisav4, Ahmet Süha Yalçın1, 2, *
1 Department of Biochemistry, School of Medicine, Marmara University, İstanbul, Turkey;
2 Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, İstanbul, Turkey;
3 Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Kocaeli, Turkey;
4 Faculty of Medicine and Faculty of Health Science, University of Ljubljana, Ljubljana, Slovenia

Article Information


Identifiers and Pagination:

Year: 2020
Volume: 2
Issue: 1
First Page: 47
Last Page: 55
Publisher ID: CNT-2-1-47
DOI: 10.2174/2665978601999200807160528

Article History:

Received Date: 19/03/2020
Revision Received Date: 08/06/2020
Acceptance Date: 06/07/2020
Electronic publication date: 07/08/2020

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Copyright: 2021 Bentham Science Publishers

* Address correspondence to this author at the Department of Biochemistry, School of Medicine, Marmara University, İstanbul, Turkey and Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, İstanbul, Turkey; Tel: ++90-216-777-5617; E-mail: asyalcin@marmara.edu.tr


Abstract

Background: Different cellular responses influence the progress of cancer. In this study, the effects of hydrogen peroxide and quercetin induced changes on cell viability, apoptosis, and oxidative stress in human hepatocellular carcinoma (HepG2) cells were investigated.

Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases, and oxidative stress related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes, and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50, and proteasome activity were determined by Western blot and fluorometry, respectively.

Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment.

Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.

Keywords: HepG2 cells, oxidative stress, hydrogen peroxide, quercetin, apoptosis, cell cycle.